TB-500 Reconstitution & Dosage Calculator
A Thymosin Beta-4 fragment studied for cell migration, angiogenesis, and tissue repair.
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How to reconstitute TB-500
TR8CE uses one simple standard: 0.1 mL of bacteriostatic water per mg. A 5 mg vial takes 0.5 mL of water for a clean 10 mg/mL solution. Larger vials scale the water up to fit the glass.
Mixing is one decision: how much bacteriostatic water goes in. Dosing follows from it, and the syringe reads the result. The instructions below are the whole procedure.
| Vial size | BAC water | Concentration | 2 mg draws to |
|---|---|---|---|
| 5 mg | 0.5 mL | 10 mg/mL | 20 units |
| 10 mg | 1 mL | 10 mg/mL | 20 units |
Units are shown for a U-100 insulin syringe. A common starting dose is 2 mg. At 10 mg/mL, that draws to 20 units on a U-100 insulin syringe.
How TB-500 works
Regulates actin binding to promote cell migration and angiogenesis while reducing inflammation. Biological activity may persist beyond its measured half-life.
Reported side effects
- Fatigue
- Headaches
- Lethargy post-injection
When is TB-500 taken?
TB-500 is commonly taken in the morning.
Reconstituting TB-500, step by step
Reconstituting is the mixing step: bacteriostatic water goes into the vial of lyophilized powder, and the powder becomes a solution a syringe can measure. For a 10 mg vial of TB-500 the calculator's water volume is 1 mL, which gives 10 mg/mL. The technique is the same for every peptide; only the water volume changes.
- Prep. Remove the plastic caps from the TB-500 vial and the bacteriostatic water vial. Wipe both rubber stoppers with an alcohol pad and let them air dry for about ten seconds.
- Equalize the pressure. Pull 1 mL of air into the mixing syringe and push it into the water vial. The positive pressure makes the draw easier.
- Draw the water. Turn the water vial upside down and draw 1 mL.
- Inject down the glass. Put the needle through the center of the TB-500 stopper, aim the tip at the inside wall, and press slowly so the water runs down the glass instead of hitting the powder. Peptides are fragile chains, and a jet of water straight onto the powder can damage them.
- Swirl, never shake. Withdraw the syringe and roll the vial in slow circles until the solution is clear.
The reasoning behind each step is in the guide Peptide reconstitution is one number. Bacteriostatic water, not sterile water, is what makes a multi-draw vial possible; the difference, and the 28-day question, is in bacteriostatic vs sterile water.
Dilution: the water volume sets the units
Two numbers decide everything on the syringe. Concentration is the vial's mg divided by the water added, and the draw is the dose divided by that concentration, read as units on a U-100 insulin syringe, where 1 mL is 100 units. Diluting a vial with more water does not change the amount of TB-500 in it; it spreads the same 10 mg across more liquid, so every dose becomes a larger draw. Less water does the opposite.
| Water added to 10 mg | Concentration | 2 mg draws to |
|---|---|---|
| 0.5 mL | 20 mg/mL | 10 units |
| 1 mL (the standard) | 10 mg/mL | 20 units |
| 2 mL | 5 mg/mL | 40 units |
The standard row is what the calculator uses for this vial. The other rows show the arithmetic, not a recommendation. The dose is entered in the calculator, never read off a table.
How many vials a 10 mL bottle of bacteriostatic water mixes
Bacteriostatic water is sold in 10 mL and 30 mL bottles. At 1 mL per 10 mg vial of TB-500, a 10 mL bottle reconstitutes 10 vials, and a 30 mL bottle reconstitutes 30. The 0.9% benzyl alcohol preservative is what lets one bottle serve several vials over several weeks. What a milligram costs right now across the stores TR8CE tracks is on the peptide price index.
| Vial size | Water per vial | Vials per 10 mL bottle | Vials per 30 mL bottle |
|---|---|---|---|
| 5 mg | 0.5 mL | 20 | 60 |
| 10 mg | 1 mL | 10 | 30 |
TB-500 in units on a U-100 syringe
At 10 mg/mL, every 1 mg is 10 units. The rows below are the conversion at the calculator's standard concentration, up to one full 100-unit syringe. A vial size not listed follows the same rule: water equals size divided by 10.
| Amount of TB-500 | U-100 syringe |
|---|---|
| 1 mg | 10 units |
| 2 mg (the COMMON reference) | 20 units |
| 2.5 mg | 25 units |
| 5 mg | 50 units |
| 10 mg | 100 units |
Storage after reconstituting
Once mixed, a TB-500 vial is kept refrigerated at 36 to 46 °F (2 to 8 °C) and is not frozen. TR8CE's vault uses a common 28-day refrigerated use-by window as general research guidance, not medical advice. Most peptides reconstitute clear, and a solution that stays cloudy or shows particles after a few minutes of gentle swirling may be degraded or low purity, which is exactly the question a certificate of analysis answers before the vial is ever opened.
Frequently asked
How much bacteriostatic water do I add to TB-500?
Add 0.5 mL of bacteriostatic water to a 5 mg TB-500 vial for a 10 mg/mL solution.
What is a common TB-500 dose?
A common TB-500 starting dose is 2 mg, which at 10 mg/mL draws to 20 units on a U-100 insulin syringe.
How do you reconstitute TB-500?
Draw bacteriostatic water into a syringe, inject it slowly down the inside glass of the TB-500 vial (not onto the powder), and swirl gently until clear. Store refrigerated and use within the common window.
How do you reconstitute a 5 mg TB-500 vial?
Add 0.5 mL of bacteriostatic water to a 5 mg TB-500 vial for a 10 mg/mL solution. A 2 mg dose then draws to 20 units on a U-100 insulin syringe.
How do you reconstitute a 10 mg TB-500 vial?
Add 1 mL of bacteriostatic water to a 10 mg TB-500 vial for a 10 mg/mL solution. A 2 mg dose then draws to 20 units on a U-100 insulin syringe.
How many vials of TB-500 does a 10 mL bottle of bacteriostatic water reconstitute?
At 1 mL per 10 mg vial, a 10 mL bottle of bacteriostatic water reconstitutes 10 vials, and a 30 mL bottle reconstitutes 30.
What happens if you add more water when reconstituting TB-500?
The amount of TB-500 in the vial does not change; the concentration falls and each dose becomes a larger draw. 10 mg in 1 mL is 10 mg/mL, so 2 mg draws to 20 units; in 2 mL it is 5 mg/mL and the same 2 mg draws to 40 units.
How is reconstituted TB-500 stored?
Refrigerated at 36 to 46 °F (2 to 8 °C), never frozen, with a common 28-day refrigerated use-by window as general research guidance, not medical advice.
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