AOD-9604 Reconstitution & Dosage Calculator
An HGH fragment analog studied for fat metabolism through lipolysis without growth promotion.
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How to reconstitute AOD-9604
AOD-9604 is one of the few compounds that sits off the flat 0.1 mL per mg standard. Published protocols converge on about 2 mL of bacteriostatic water for a 5 mg vial, which lands at 2.5 mg/mL, and the water scales with the vial so the concentration stays the same at every size. These are the volumes the published protocols converge on (surveyed 2026-08-29), not a house rule.
Mixing is one decision: how much bacteriostatic water goes in. Dosing follows from it, and the syringe reads the result. The instructions below are the whole procedure.
| Vial size | BAC water | Concentration | 250 mcg draws to |
|---|---|---|---|
| 2 mg | 0.8 mL | 2.5 mg/mL | 10 units |
| 5 mg | 2 mL | 2.5 mg/mL | 10 units |
Units are shown for a U-100 insulin syringe. A common starting dose is 250 mcg. At 2.5 mg/mL, that draws to 10 units on a U-100 insulin syringe.
How AOD-9604 works
Mimics the fat-burning region of human growth hormone, stimulating the breakdown of triglycerides and suppressing fat storage. It targets lipolysis independent of insulin pathways.
Reported side effects
- Mild injection site irritation
- Rare headaches
- Transient fatigue
When is AOD-9604 taken?
AOD-9604 is commonly taken in the morning.
Reconstituting AOD-9604, step by step
Reconstituting is the mixing step: bacteriostatic water goes into the vial of lyophilized powder, and the powder becomes a solution a syringe can measure. For a 5 mg vial of AOD-9604 the calculator's water volume is 2 mL, which gives 2.5 mg/mL. The technique is the same for every peptide; only the water volume changes.
- Prep. Remove the plastic caps from the AOD-9604 vial and the bacteriostatic water vial. Wipe both rubber stoppers with an alcohol pad and let them air dry for about ten seconds.
- Equalize the pressure. Pull 2 mL of air into the mixing syringe and push it into the water vial. The positive pressure makes the draw easier.
- Draw the water. Turn the water vial upside down and draw 2 mL.
- Inject down the glass. Put the needle through the center of the AOD-9604 stopper, aim the tip at the inside wall, and press slowly so the water runs down the glass instead of hitting the powder. Peptides are fragile chains, and a jet of water straight onto the powder can damage them.
- Swirl, never shake. Withdraw the syringe and roll the vial in slow circles until the solution is clear.
The reasoning behind each step is in the guide Peptide reconstitution is one number. Bacteriostatic water, not sterile water, is what makes a multi-draw vial possible; the difference, and the 28-day question, is in bacteriostatic vs sterile water.
Dilution: the water volume sets the units
Two numbers decide everything on the syringe. Concentration is the vial's mg divided by the water added, and the draw is the dose divided by that concentration, read as units on a U-100 insulin syringe, where 1 mL is 100 units. Diluting a vial with more water does not change the amount of AOD-9604 in it; it spreads the same 5 mg across more liquid, so every dose becomes a larger draw. Less water does the opposite.
| Water added to 5 mg | Concentration | 250 mcg draws to |
|---|---|---|
| 1 mL | 5 mg/mL | 5 units |
| 2 mL (the standard) | 2.5 mg/mL | 10 units |
| 4 mL | 1.25 mg/mL | 20 units |
The standard row is what the calculator uses for this vial. The other rows show the arithmetic, not a recommendation. The dose is entered in the calculator, never read off a table.
How many vials a 10 mL bottle of bacteriostatic water mixes
Bacteriostatic water is sold in 10 mL and 30 mL bottles. At 2 mL per 5 mg vial of AOD-9604, a 10 mL bottle reconstitutes 5 vials, and a 30 mL bottle reconstitutes 15. The 0.9% benzyl alcohol preservative is what lets one bottle serve several vials over several weeks. What a milligram costs right now across the stores TR8CE tracks is on the peptide price index.
| Vial size | Water per vial | Vials per 10 mL bottle | Vials per 30 mL bottle |
|---|---|---|---|
| 2 mg | 0.8 mL | 12 | 37 |
| 5 mg | 2 mL | 5 | 15 |
AOD-9604 in units on a U-100 syringe
At 2.5 mg/mL, every 1 mg is 40 units. The rows below are the conversion at the calculator's standard concentration, up to one full 100-unit syringe. A vial size not listed follows the same rule: water equals size divided by 2.5.
| Amount of AOD-9604 | U-100 syringe |
|---|---|
| 50 mcg | 2 units |
| 100 mcg | 4 units |
| 200 mcg | 8 units |
| 250 mcg (the COMMON reference) | 10 units |
| 500 mcg | 20 units |
| 2.5 mg | 100 units |
Storage after reconstituting
Once mixed, a AOD-9604 vial is kept refrigerated at 36 to 46 °F (2 to 8 °C) and is not frozen. TR8CE's vault uses a common 28-day refrigerated use-by window as general research guidance, not medical advice. Most peptides reconstitute clear, and a solution that stays cloudy or shows particles after a few minutes of gentle swirling may be degraded or low purity, which is exactly the question a certificate of analysis answers before the vial is ever opened.
Frequently asked
How much bacteriostatic water do I add to AOD-9604?
Add 0.8 mL of bacteriostatic water to a 2 mg AOD-9604 vial for a 2.5 mg/mL solution. A 5 mg vial takes 2 mL.
What is a common AOD-9604 dose?
A common AOD-9604 starting dose is 250 mcg, which at 2.5 mg/mL draws to 10 units on a U-100 insulin syringe.
How do you reconstitute AOD-9604?
Draw bacteriostatic water into a syringe, inject it slowly down the inside glass of the AOD-9604 vial (not onto the powder), and swirl gently until clear. Store refrigerated and use within the common window.
How do you reconstitute a 2 mg AOD-9604 vial?
Add 0.8 mL of bacteriostatic water to a 2 mg AOD-9604 vial for a 2.5 mg/mL solution. A 250 mcg dose then draws to 10 units on a U-100 insulin syringe.
How do you reconstitute a 5 mg AOD-9604 vial?
Add 2 mL of bacteriostatic water to a 5 mg AOD-9604 vial for a 2.5 mg/mL solution. A 250 mcg dose then draws to 10 units on a U-100 insulin syringe.
How many vials of AOD-9604 does a 10 mL bottle of bacteriostatic water reconstitute?
At 2 mL per 5 mg vial, a 10 mL bottle of bacteriostatic water reconstitutes 5 vials, and a 30 mL bottle reconstitutes 15.
What happens if you add more water when reconstituting AOD-9604?
The amount of AOD-9604 in the vial does not change; the concentration falls and each dose becomes a larger draw. 5 mg in 2 mL is 2.5 mg/mL, so 250 mcg draws to 10 units; in 4 mL it is 1.25 mg/mL and the same 250 mcg draws to 20 units.
How is reconstituted AOD-9604 stored?
Refrigerated at 36 to 46 °F (2 to 8 °C), never frozen, with a common 28-day refrigerated use-by window as general research guidance, not medical advice.
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