Semaglutide Reconstitution & Dosage Calculator
Once-weekly GLP-1 receptor agonist studied for glucose control and weight loss.
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How to reconstitute Semaglutide
TR8CE uses one simple standard: 0.1 mL of bacteriostatic water per mg. A 5 mg vial takes 0.5 mL of water for a clean 10 mg/mL solution. Larger vials scale the water up to fit the glass.
Mixing is one decision: how much bacteriostatic water goes in. Dosing follows from it, and the syringe reads the result. The instructions below are the whole procedure.
| Vial size | BAC water | Concentration | 0.25 mg draws to |
|---|---|---|---|
| 5 mg | 0.5 mL | 10 mg/mL | 2.5 units |
| 10 mg | 1 mL | 10 mg/mL | 2.5 units |
| 15 mg | 1.5 mL | 10 mg/mL | 2.5 units |
| 20 mg | 2 mL | 10 mg/mL | 2.5 units |
| 30 mg | 3 mL | 10 mg/mL | 2.5 units |
Units are shown for a U-100 insulin syringe. A common starting dose is 0.25 mg. At 10 mg/mL, that draws to 2.5 units on a U-100 insulin syringe.
How many units is my Semaglutide dose?
A listing that says 10 mL is naming the vial's liquid capacity, not the peptide inside it. The mass is the number in milligrams on the label, and the concentration is decided by the water you add: a 10 mg vial is 10 mg/mL with 1 mL of bacteriostatic water and 1 mg/mL with 10 mL. Find your vial and the water you added, then read across. Units are the marks on a U-100 insulin syringe, where 100 units is 1 mL.
| Vial | BAC water | Concentration | 0.25 mg draws to | 0.5 mg draws to | 1 mg draws to |
|---|---|---|---|---|---|
| 5 mg | 1 mL | 5 mg/mL | 0.05 mL (5 u) | 0.1 mL (10 u) | 0.2 mL (20 u) |
| 5 mg | 2 mL | 2.5 mg/mL | 0.1 mL (10 u) | 0.2 mL (20 u) | 0.4 mL (40 u) |
| 10 mg | 1 mL | 10 mg/mL | 0.03 mL (2.5 u) | 0.05 mL (5 u) | 0.1 mL (10 u) |
| 10 mg | 2 mL | 5 mg/mL | 0.05 mL (5 u) | 0.1 mL (10 u) | 0.2 mL (20 u) |
| 10 mg | 5 mL | 2 mg/mL | 0.12 mL (12.5 u) | 0.25 mL (25 u) | 0.5 mL (50 u) |
| 10 mg | 10 mL | 1 mg/mL | 0.25 mL (25 u) | 0.5 mL (50 u) | 1 mL (100 u) |
| 20 mg | 2 mL | 10 mg/mL | 0.03 mL (2.5 u) | 0.05 mL (5 u) | 0.1 mL (10 u) |
| 30 mg | 3 mL | 10 mg/mL | 0.03 mL (2.5 u) | 0.05 mL (5 u) | 0.1 mL (10 u) |
These are conversions, not a protocol. A U-100 syringe holds 100 units, so anything past that is drawn as more than one syringe or measured in mL.
How Semaglutide works
Single-pathway GLP-1 receptor agonism enhances glucose-dependent insulin secretion, suppresses appetite, and delays gastric emptying.
Reported side effects
- Nausea
- Constipation
- Headache
- Decreased appetite
When is Semaglutide taken?
Semaglutide is commonly taken in the morning.
Reconstituting Semaglutide, step by step
Reconstituting is the mixing step: bacteriostatic water goes into the vial of lyophilized powder, and the powder becomes a solution a syringe can measure. For a 5 mg vial of Semaglutide the calculator's water volume is 0.5 mL, which gives 10 mg/mL. The technique is the same for every peptide; only the water volume changes.
- Prep. Remove the plastic caps from the Semaglutide vial and the bacteriostatic water vial. Wipe both rubber stoppers with an alcohol pad and let them air dry for about ten seconds.
- Equalize the pressure. Pull 0.5 mL of air into the mixing syringe and push it into the water vial. The positive pressure makes the draw easier.
- Draw the water. Turn the water vial upside down and draw 0.5 mL.
- Inject down the glass. Put the needle through the center of the Semaglutide stopper, aim the tip at the inside wall, and press slowly so the water runs down the glass instead of hitting the powder. Peptides are fragile chains, and a jet of water straight onto the powder can damage them.
- Swirl, never shake. Withdraw the syringe and roll the vial in slow circles until the solution is clear.
The reasoning behind each step is in the guide Peptide reconstitution is one number. Bacteriostatic water, not sterile water, is what makes a multi-draw vial possible; the difference, and the 28-day question, is in bacteriostatic vs sterile water.
Dilution: the water volume sets the units
Two numbers decide everything on the syringe. Concentration is the vial's mg divided by the water added, and the draw is the dose divided by that concentration, read as units on a U-100 insulin syringe, where 1 mL is 100 units. Diluting a vial with more water does not change the amount of Semaglutide in it; it spreads the same 5 mg across more liquid, so every dose becomes a larger draw. Less water does the opposite.
| Water added to 5 mg | Concentration | 0.25 mg draws to |
|---|---|---|
| 0.25 mL | 20 mg/mL | 1.25 units |
| 0.5 mL (the standard) | 10 mg/mL | 2.5 units |
| 1 mL | 5 mg/mL | 5 units |
The standard row is what the calculator uses for this vial. The other rows show the arithmetic, not a recommendation. The dose is entered in the calculator, never read off a table.
How many vials a 10 mL bottle of bacteriostatic water mixes
Bacteriostatic water is sold in 10 mL and 30 mL bottles. At 0.5 mL per 5 mg vial of Semaglutide, a 10 mL bottle reconstitutes 20 vials, and a 30 mL bottle reconstitutes 60. The 0.9% benzyl alcohol preservative is what lets one bottle serve several vials over several weeks. What a milligram costs right now across the stores TR8CE tracks is on the peptide price index.
| Vial size | Water per vial | Vials per 10 mL bottle | Vials per 30 mL bottle |
|---|---|---|---|
| 5 mg | 0.5 mL | 20 | 60 |
| 10 mg | 1 mL | 10 | 30 |
| 15 mg | 1.5 mL | 6 | 20 |
| 20 mg | 2 mL | 5 | 15 |
| 30 mg | 3 mL | 3 | 10 |
Semaglutide in units on a U-100 syringe
At 10 mg/mL, every 1 mg is 10 units. The rows below are the conversion at the calculator's standard concentration, up to one full 100-unit syringe. A vial size not listed follows the same rule: water equals size divided by 10.
| Amount of Semaglutide | U-100 syringe |
|---|---|
| 0.25 mg (the COMMON reference) | 2.5 units |
| 1 mg | 10 units |
| 2 mg | 20 units |
| 2.5 mg | 25 units |
Storage after reconstituting
Once mixed, a Semaglutide vial is kept refrigerated at 36 to 46 °F (2 to 8 °C) and is not frozen. TR8CE's vault uses a common 28-day refrigerated use-by window as general research guidance, not medical advice. Most peptides reconstitute clear, and a solution that stays cloudy or shows particles after a few minutes of gentle swirling may be degraded or low purity, which is exactly the question a certificate of analysis answers before the vial is ever opened.
Frequently asked
How much bacteriostatic water do I add to Semaglutide?
Add 0.5 mL of bacteriostatic water to a 5 mg Semaglutide vial for a 10 mg/mL solution.
What is a common Semaglutide dose?
A common Semaglutide starting dose is 0.25 mg, which at 10 mg/mL draws to 2.5 units on a U-100 insulin syringe.
How do you reconstitute Semaglutide?
Draw bacteriostatic water into a syringe, inject it slowly down the inside glass of the Semaglutide vial (not onto the powder), and swirl gently until clear. Store refrigerated and use within the common window.
How do you reconstitute a 5 mg Semaglutide vial?
Add 0.5 mL of bacteriostatic water to a 5 mg Semaglutide vial for a 10 mg/mL solution. A 0.25 mg dose then draws to 2.5 units on a U-100 insulin syringe.
How do you reconstitute a 10 mg Semaglutide vial?
Add 1 mL of bacteriostatic water to a 10 mg Semaglutide vial for a 10 mg/mL solution. A 0.25 mg dose then draws to 2.5 units on a U-100 insulin syringe.
How do you reconstitute a 15 mg Semaglutide vial?
Add 1.5 mL of bacteriostatic water to a 15 mg Semaglutide vial for a 10 mg/mL solution. A 0.25 mg dose then draws to 2.5 units on a U-100 insulin syringe.
How do you reconstitute a 20 mg Semaglutide vial?
Add 2 mL of bacteriostatic water to a 20 mg Semaglutide vial for a 10 mg/mL solution. A 0.25 mg dose then draws to 2.5 units on a U-100 insulin syringe.
How do you reconstitute a 30 mg Semaglutide vial?
Add 3 mL of bacteriostatic water to a 30 mg Semaglutide vial for a 10 mg/mL solution. A 0.25 mg dose then draws to 2.5 units on a U-100 insulin syringe.
What does 10 mL semaglutide mean?
It describes the vial, not the dose. A 10 mL vial is the glass; the peptide in it is whatever the label says in milligrams. Fill a 10 mg vial with 10 mL of bacteriostatic water and you have 1 mg/mL, where a 0.25 mg draw is 0.25 mL, or 25 units on a U-100 insulin syringe. The same vial with 1 mL of water is 10 mg/mL, where that draw is 2.5 units.
How much bacteriostatic water for a 10 mg semaglutide vial?
1 mL gives 10 mg/mL, which is the TR8CE standard and puts a 0.25 mg draw at 2.5 units on a U-100 syringe. More water lowers the concentration and moves the same dose further up the barrel, which is easier to read but empties the vial no faster.
How many vials of Semaglutide does a 10 mL bottle of bacteriostatic water reconstitute?
At 0.5 mL per 5 mg vial, a 10 mL bottle of bacteriostatic water reconstitutes 20 vials, and a 30 mL bottle reconstitutes 60.
What happens if you add more water when reconstituting Semaglutide?
The amount of Semaglutide in the vial does not change; the concentration falls and each dose becomes a larger draw. 5 mg in 0.5 mL is 10 mg/mL, so 0.25 mg draws to 2.5 units; in 1 mL it is 5 mg/mL and the same 0.25 mg draws to 5 units.
How is reconstituted Semaglutide stored?
Refrigerated at 36 to 46 °F (2 to 8 °C), never frozen, with a common 28-day refrigerated use-by window as general research guidance, not medical advice.
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