Glutathione Reconstitution & Dosage Calculator
The body's master antioxidant tripeptide, used for ROS neutralization and phase II detoxification support.
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How to reconstitute Glutathione
Glutathione reconstitutes to a set concentration. Add 5 mL of bacteriostatic water to a 500 mg vial for a 100 mg/mL solution.
Mixing is one decision: how much bacteriostatic water goes in. Dosing follows from it, and the syringe reads the result. The instructions below are the whole procedure.
| Vial size | BAC water | Concentration | 100 mg draws to |
|---|---|---|---|
| 500 mg | 5 mL | 100 mg/mL | 100 units |
Units are shown for a U-100 insulin syringe. A common starting dose is 100 mg. At 100 mg/mL, that draws to 100 units on a U-100 insulin syringe.
How many units is my Glutathione dose?
Glutathione ships in vials from 500 mg to 1500 mg, and the syringe reads from the concentration you made, not from the label. A 1500 mg vial is 150 mg/mL with 10 mL of bacteriostatic water and 100 mg/mL with 15 mL. Find your vial and the water you added, then read across. Units are the marks on a U-100 insulin syringe, where 100 units is 1 mL.
| Vial | BAC water | Concentration | 50 mg draws to | 100 mg draws to | 200 mg draws to |
|---|---|---|---|---|---|
| 500 mg | 5 mL | 100 mg/mL | 0.5 mL (50 u) | 1 mL (100 u) | 2 mL (200 u) |
| 500 mg | 10 mL | 50 mg/mL | 1 mL (100 u) | 2 mL (200 u) | 4 mL (400 u) |
| 600 mg | 5 mL | 120 mg/mL | 0.42 mL (41.67 u) | 0.83 mL (83.33 u) | 1.67 mL (166.67 u) |
| 600 mg | 6 mL | 100 mg/mL | 0.5 mL (50 u) | 1 mL (100 u) | 2 mL (200 u) |
| 1200 mg | 10 mL | 120 mg/mL | 0.42 mL (41.67 u) | 0.83 mL (83.33 u) | 1.67 mL (166.67 u) |
| 1200 mg | 12 mL | 100 mg/mL | 0.5 mL (50 u) | 1 mL (100 u) | 2 mL (200 u) |
| 1500 mg | 10 mL | 150 mg/mL | 0.33 mL (33.33 u) | 0.67 mL (66.67 u) | 1.33 mL (133.33 u) |
| 1500 mg | 15 mL | 100 mg/mL | 0.5 mL (50 u) | 1 mL (100 u) | 2 mL (200 u) |
These are conversions, not a protocol. A U-100 syringe holds 100 units, so anything past that is drawn as more than one syringe or measured in mL.
How Glutathione works
Acts as an electron donor to neutralize reactive oxygen species and supports phase II conjugation via glutathione S-transferases.
Reported side effects
- Possible GI discomfort
- Rare allergic reaction
When is Glutathione taken?
Glutathione is commonly taken in the morning.
Reconstituting Glutathione, step by step
Reconstituting is the mixing step: bacteriostatic water goes into the vial of lyophilized powder, and the powder becomes a solution a syringe can measure. For a 500 mg vial of Glutathione the calculator's water volume is 5 mL, which gives 100 mg/mL. The technique is the same for every peptide; only the water volume changes.
- Prep. Remove the plastic caps from the Glutathione vial and the bacteriostatic water vial. Wipe both rubber stoppers with an alcohol pad and let them air dry for about ten seconds.
- Equalize the pressure. Pull 5 mL of air into the mixing syringe and push it into the water vial. The positive pressure makes the draw easier.
- Draw the water. Turn the water vial upside down and draw 5 mL.
- Inject down the glass. Put the needle through the center of the Glutathione stopper, aim the tip at the inside wall, and press slowly so the water runs down the glass instead of hitting the powder. Peptides are fragile chains, and a jet of water straight onto the powder can damage them.
- Swirl, never shake. Withdraw the syringe and roll the vial in slow circles until the solution is clear.
The reasoning behind each step is in the guide Peptide reconstitution is one number. Bacteriostatic water, not sterile water, is what makes a multi-draw vial possible; the difference, and the 28-day question, is in bacteriostatic vs sterile water.
Dilution: the water volume sets the units
Two numbers decide everything on the syringe. Concentration is the vial's mg divided by the water added, and the draw is the dose divided by that concentration, read as units on a U-100 insulin syringe, where 1 mL is 100 units. Diluting a vial with more water does not change the amount of Glutathione in it; it spreads the same 500 mg across more liquid, so every dose becomes a larger draw. Less water does the opposite.
| Water added to 500 mg | Concentration | 100 mg draws to |
|---|---|---|
| 2.5 mL | 200 mg/mL | 50 units |
| 5 mL (the standard) | 100 mg/mL | 100 units |
The standard row is what the calculator uses for this vial. The other rows show the arithmetic, not a recommendation. The dose is entered in the calculator, never read off a table.
How many vials a 10 mL bottle of bacteriostatic water mixes
Bacteriostatic water is sold in 10 mL and 30 mL bottles. At 5 mL per 500 mg vial of Glutathione, a 10 mL bottle reconstitutes 2 vials, and a 30 mL bottle reconstitutes 6. The 0.9% benzyl alcohol preservative is what lets one bottle serve several vials over several weeks. What a milligram costs right now across the stores TR8CE tracks is on the peptide price index.
| Vial size | Water per vial | Vials per 10 mL bottle | Vials per 30 mL bottle |
|---|---|---|---|
| 500 mg | 5 mL | 2 | 6 |
Glutathione in units on a U-100 syringe
At 100 mg/mL, every 1 mg is 1 unit. The rows below are the conversion at the calculator's standard concentration, up to one full 100-unit syringe. A vial size not listed follows the same rule: water equals size divided by 100.
| Amount of Glutathione | U-100 syringe |
|---|---|
| 50 mg | 50 units |
| 100 mg (the COMMON reference) | 100 units |
Storage after reconstituting
Once mixed, a Glutathione vial is kept refrigerated at 36 to 46 °F (2 to 8 °C) and is not frozen. TR8CE's vault uses a common 28-day refrigerated use-by window as general research guidance, not medical advice. Most peptides reconstitute clear, and a solution that stays cloudy or shows particles after a few minutes of gentle swirling may be degraded or low purity, which is exactly the question a certificate of analysis answers before the vial is ever opened.
Frequently asked
How much bacteriostatic water do I add to Glutathione?
Add 5 mL of bacteriostatic water to a 500 mg Glutathione vial for a 100 mg/mL solution.
What is a common Glutathione dose?
A common Glutathione starting dose is 100 mg, which at 100 mg/mL draws to 100 units on a U-100 insulin syringe.
How do you reconstitute Glutathione?
Draw bacteriostatic water into a syringe, inject it slowly down the inside glass of the Glutathione vial (not onto the powder), and swirl gently until clear. Store refrigerated and use within the common window.
How do you reconstitute a 500 mg Glutathione vial?
Add 5 mL of bacteriostatic water to a 500 mg Glutathione vial for a 100 mg/mL solution. A 100 mg dose then draws to 100 units on a U-100 insulin syringe.
How do you reconstitute a 1500 mg glutathione vial?
It depends on the volume you add. 10 mL of bacteriostatic water gives 150 mg/mL, so a 100 mg draw is 0.67 mL, which is 67 units on a U-100 insulin syringe. 15 mL gives 100 mg/mL, where the same 100 mg draw is a full 1 mL, or 100 units.
How many units is 100 mg of glutathione?
At 100 mg/mL it is 1 mL, which is 100 units on a U-100 insulin syringe. At 150 mg/mL it is 0.67 mL, or 67 units. At 300 mg/mL it is 0.33 mL, or 33 units.
How many vials of Glutathione does a 10 mL bottle of bacteriostatic water reconstitute?
At 5 mL per 500 mg vial, a 10 mL bottle of bacteriostatic water reconstitutes 2 vials, and a 30 mL bottle reconstitutes 6.
What happens if you add more water when reconstituting Glutathione?
The amount of Glutathione in the vial does not change; the concentration falls and each dose becomes a larger draw. 500 mg in 5 mL is 100 mg/mL, so 100 mg draws to 100 units; in 10 mL it is 50 mg/mL and the same 100 mg draws to 200 units.
How is reconstituted Glutathione stored?
Refrigerated at 36 to 46 °F (2 to 8 °C), never frozen, with a common 28-day refrigerated use-by window as general research guidance, not medical advice.
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