NAD+ Reconstitution & Dosage Calculator
A coenzyme central to cellular energy metabolism, studied in longevity and metabolic research.
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How to reconstitute NAD+
NAD+ reconstitutes to a set concentration. Add 5 mL of bacteriostatic water to a 500 mg vial for a 100 mg/mL solution.
Mixing is one decision: how much bacteriostatic water goes in. Dosing follows from it, and the syringe reads the result. The instructions below are the whole procedure.
| Vial size | BAC water | Concentration | 25 mg draws to |
|---|---|---|---|
| 500 mg | 5 mL | 100 mg/mL | 25 units |
| 1000 mg | 10 mL | 100 mg/mL | 25 units |
Units are shown for a U-100 insulin syringe. A common starting dose is 25 mg. At 100 mg/mL, that draws to 25 units on a U-100 insulin syringe.
How NAD+ works
Acts as an essential coenzyme that shuttles electrons in energy production and fuels enzymes involved in DNA repair and cellular maintenance. Research interest centers on restoring levels that decline with age.
Reported side effects
- Flushing
- Mild nausea
- Headache at high doses
When is NAD+ taken?
NAD+ is commonly taken in the morning.
Reconstituting NAD+, step by step
Reconstituting is the mixing step: bacteriostatic water goes into the vial of lyophilized powder, and the powder becomes a solution a syringe can measure. For a 500 mg vial of NAD+ the calculator's water volume is 5 mL, which gives 100 mg/mL. The technique is the same for every peptide; only the water volume changes.
- Prep. Remove the plastic caps from the NAD+ vial and the bacteriostatic water vial. Wipe both rubber stoppers with an alcohol pad and let them air dry for about ten seconds.
- Equalize the pressure. Pull 5 mL of air into the mixing syringe and push it into the water vial. The positive pressure makes the draw easier.
- Draw the water. Turn the water vial upside down and draw 5 mL.
- Inject down the glass. Put the needle through the center of the NAD+ stopper, aim the tip at the inside wall, and press slowly so the water runs down the glass instead of hitting the powder. Peptides are fragile chains, and a jet of water straight onto the powder can damage them.
- Swirl, never shake. Withdraw the syringe and roll the vial in slow circles until the solution is clear.
The reasoning behind each step is in the guide Peptide reconstitution is one number. Bacteriostatic water, not sterile water, is what makes a multi-draw vial possible; the difference, and the 28-day question, is in bacteriostatic vs sterile water.
Dilution: the water volume sets the units
Two numbers decide everything on the syringe. Concentration is the vial's mg divided by the water added, and the draw is the dose divided by that concentration, read as units on a U-100 insulin syringe, where 1 mL is 100 units. Diluting a vial with more water does not change the amount of NAD+ in it; it spreads the same 500 mg across more liquid, so every dose becomes a larger draw. Less water does the opposite.
| Water added to 500 mg | Concentration | 25 mg draws to |
|---|---|---|
| 2.5 mL | 200 mg/mL | 12.5 units |
| 5 mL (the standard) | 100 mg/mL | 25 units |
| 10 mL | 50 mg/mL | 50 units |
The standard row is what the calculator uses for this vial. The other rows show the arithmetic, not a recommendation. The dose is entered in the calculator, never read off a table.
How many vials a 10 mL bottle of bacteriostatic water mixes
Bacteriostatic water is sold in 10 mL and 30 mL bottles. At 5 mL per 500 mg vial of NAD+, a 10 mL bottle reconstitutes 2 vials, and a 30 mL bottle reconstitutes 6. The 0.9% benzyl alcohol preservative is what lets one bottle serve several vials over several weeks. What a milligram costs right now across the stores TR8CE tracks is on the peptide price index.
| Vial size | Water per vial | Vials per 10 mL bottle | Vials per 30 mL bottle |
|---|---|---|---|
| 500 mg | 5 mL | 2 | 6 |
| 1000 mg | 10 mL | 1 | 3 |
NAD+ in units on a U-100 syringe
At 100 mg/mL, every 1 mg is 1 unit. The rows below are the conversion at the calculator's standard concentration, up to one full 100-unit syringe. A vial size not listed follows the same rule: water equals size divided by 100.
| Amount of NAD+ | U-100 syringe |
|---|---|
| 5 mg | 5 units |
| 10 mg | 10 units |
| 20 mg | 20 units |
| 25 mg (the COMMON reference) | 25 units |
| 50 mg | 50 units |
| 100 mg | 100 units |
Storage after reconstituting
Once mixed, a NAD+ vial is kept refrigerated at 36 to 46 °F (2 to 8 °C) and is not frozen. TR8CE's vault uses a common 28-day refrigerated use-by window as general research guidance, not medical advice. Most peptides reconstitute clear, and a solution that stays cloudy or shows particles after a few minutes of gentle swirling may be degraded or low purity, which is exactly the question a certificate of analysis answers before the vial is ever opened.
NAD+ and NMN are not the same vial
NAD+ (nicotinamide adenine dinucleotide, written with the plus for its oxidized form) is the molecule in this vial. NMN (nicotinamide mononucleotide) is a different, smaller molecule, a precursor the body converts into NAD+. The water volumes and unit conversions on this page are for NAD+ vials only. An NMN vial is a different compound with its own label and its own math.
Frequently asked
How much bacteriostatic water do I add to NAD+?
Add 5 mL of bacteriostatic water to a 500 mg NAD+ vial for a 100 mg/mL solution.
What is a common NAD+ dose?
A common NAD+ starting dose is 25 mg, which at 100 mg/mL draws to 25 units on a U-100 insulin syringe.
How do you reconstitute NAD+?
Draw bacteriostatic water into a syringe, inject it slowly down the inside glass of the NAD+ vial (not onto the powder), and swirl gently until clear. Store refrigerated and use within the common window.
How do you reconstitute a 500 mg NAD+ vial?
Add 5 mL of bacteriostatic water to a 500 mg NAD+ vial for a 100 mg/mL solution. A 25 mg dose then draws to 25 units on a U-100 insulin syringe.
How do you reconstitute a 1000 mg NAD+ vial?
Add 10 mL of bacteriostatic water to a 1000 mg NAD+ vial for a 100 mg/mL solution. A 25 mg dose then draws to 25 units on a U-100 insulin syringe.
How many vials of NAD+ does a 10 mL bottle of bacteriostatic water reconstitute?
At 5 mL per 500 mg vial, a 10 mL bottle of bacteriostatic water reconstitutes 2 vials, and a 30 mL bottle reconstitutes 6.
What happens if you add more water when reconstituting NAD+?
The amount of NAD+ in the vial does not change; the concentration falls and each dose becomes a larger draw. 500 mg in 5 mL is 100 mg/mL, so 25 mg draws to 25 units; in 10 mL it is 50 mg/mL and the same 25 mg draws to 50 units.
How is reconstituted NAD+ stored?
Refrigerated at 36 to 46 °F (2 to 8 °C), never frozen, with a common 28-day refrigerated use-by window as general research guidance, not medical advice.
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